A multi-strategy antimicrobial discovery approach reveals new ways to combat Chla­mydia. Here, we describe a multi-strategy screening campaign carried out to probe the chemical space for novel selective antichlamydial activities. Over sixty potent compounds dissimilar to known antibiotics were identified. These may now serve as starting points for developing more sustainable therapeutics and as tools for dissecting the unique biology of Chlamydia. The project was primarily driven by Magnus Ölander, a terrific postdoc in the Sixt lab, yet was also a team effort involving important contributions from several lab members, as well as from our collaborators in the lab of Björn Schröder.